Ain 76a diet
Because of these differences, it became quite difficult to compare observations across these nutrient study disciplines, from lab group to lab group. For example, Vitamin A researchers developed separate and very distinct purified diet formulas from those studying Vitamin D or selenium or Vitamin E. Carbohydrates in this case are supplied by corn starch and sucrose, corn oil provides the fat and cellulose supplies the fiber. The AIN-76A rodent diet formula was the result.
(It is true that casein, for example contains trace levels of certain vitamins and will contain small amounts of some minerals. Vitamin and mineral mixes specific to rodents are added to ensure adequacy. In the AIN-76A rodent diet, the protein requirement is met by the milk protein casein, along with added methionine (to meet sulfur-containing amino acid requirements). Despite these differences, the formulas were generally well reported, allowing one group to know exactly what another group had fed their animals. Each nutrient is supplied by a separate, purified ingredient.
